Recomb inant Human Tumor Necrosis Factor beta

名称：Recomb inant Human Tumor Necrosis Factor beta

货号：UN072

规格：5μg/20μg/50μg/100μg/500μg/1mg

纯度：>96%

系统：E.coli.

产品详情

Recombinant Human Tumor Necrosis Factor beta

TNF-β is a potent mediator of inflammatory and immune responses. It belongs to the TNF family of ligands, and signals through TNFR1 and TNFR2. TNF-β is produced by activated T and B lymphocytes, and has similar activities to TNF-α. Like TNF-α, TNF-β is involved in the regulation of various biological processes, including cell proliferation, differentiation, apoptosis, lipid metabolism, coagulation, and neurotransmission. TNF-β is secreted as a soluble polypeptide, but can form heterotrimers with lymphotoxin-β, which effectively anchors the TNF-β to the cell surface. TNF-β is cytotoxic to a wide range of tumor cells. Human TNF-beta is a 22kDa protein that shares 73% amino acid sequence identity with mouse and rat LT-alpha /TNF-beta. Recombinant Human TNF-β is a secreted 171 amino acid protein (18.7kDa) which forms an active non-disulfide-linked homotrimer structure in solution.

 名称缩写/Abbreviation：

rHuTNF-β

货号/Item No.：

UN072

别名/Synonyms：

Tumor Necrosis Factor-β, TNFSF1B, Lymphotoxin-alpha (LT-α)

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 18.7 kDa, a polypeptide chain containing 171 amino acids. The native form of TNF-beta is reportedly a trimer.

SCI的检索号/Accession #：

P01374 Leu35-Leu205

蛋白序列/A.A. Sequence：

LPGVGLTPSAAQTARQHPKMHLAHSTLKPAAHLIGDPSKQNSLLWRANTDRAFLQDGFSLSNNSLLVPTSGIYFVYSQVVFSGKAYSPKATSSPLYLAHEVQLFSSQYPFHVPLLSSQKMVYPGLQEPWLHSMYHGAAFQLTQGDQLSTHTDGIPHLVLSPSTVFFGAFAL

纯度/Purity：

＞96% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. The ED₅₀ as determined by a cytotoxicity assay using murine L929 cells is less than 5 pg/ml, corresponding to a specific activity of ≥ 2.0×10⁸ IU/mg in the presence of actinomycin D.

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, with 0.02% (v/v) Tween-20,pH 7.4 .

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.01 EU/μg of rHuTNF-β protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.