Recombinant Human Macrophage Inf lammatory Protein-3/CCL23

名称：Recombinant Human Macrophage Inf lammatory Protein-3/CCL23

货号：UN186

规格：5μg/20μg/50μg/100μg/250μg/500μg/1mg

纯度：>97%

系统：E.coli.

产品详情

 Recombinant Human Macrophage Inflammatory Protein-3/CCL23

MIP-3 is a CC chemokine that signals through the CCR1 receptor. Highly expressed in adult lung, liver, skeletal muscle and pancreas, this protein shows strong chemotactic activity for monocytes, resting T-lymphocytes, and neutrophils, but not for activated lymphocytes. Additionally, MIP-3 has been shown to inhibit colony formation of bone marrow myeloid immature progenitors. It elicits the effects by binding to CCR1. CCL23 is reported that it can be cleaved into four forms: CCL23 (19-99) , CCL23 (22-99) , CCL23 (27-99) , CCL23 (30-99) . Recombinant Human MIP-3 is an 11.3kDa protein containing 99 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

 名称缩写/Abbreviation：

rHuMIP-3/CCL23

货号/Item No.：

UN186

别名/Synonyms：

CKB-8, MIP-3, MIPIF-1

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 11.4 kDa, a single non-glycosylated polypeptide chain containing 99 amino acids.

SCI的检索号/Accession #：

P55773 Arg24-Asn94

蛋白序列/A.A. Sequence：

RVTKDAETEFMMSKLPLENPVLLDRFHATSADCCISYTPRSIPCSLLESYFETNSECSKPGVIFLTKKGRRFCANPSDKQVQVCMRMLKLDTRIKTRKN

纯度/Purity：

＞97% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration of 10-50 ng/ml.

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, with 150 mM NaCl, pH 7.4.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.1 EU/μg of rHuMIP-3/CCL23 protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.