Recombinant Human Macrophage Inflammatory Protein-3 alpha/CCL20

名称：Recombinant Human Macrophage Inflammatory Protein-3 alpha/CCL20

货号：UN183

规格：5μg/20μg/50μg/100μg/500μg/1mg

纯度：>97%

系统：E.coli.

产品详情

 Recombinant Human Macrophage Inflammatory Protein-3 alpha/CCL20

CCL20, also named LARC (Liver and Activation-regulated Chemokine) , is encoded by CCL20 located on chromosome 2 in humans. The expression can be induced by LPS and some inflammatory cytokines, and it is at a high level in lymph nodes, PBL, fatal liver, fatal lung, appendix and so on. CCL20 has functions of chemotactic towards lymphocytes and dendritic cells. Additionally, it promotes the adhesion of memory CD4+ T cells and inhibits colony formation of bone marrow myeloid immature progenitors. CCL20 elicits it effects by binding with CCR6 receptor. Recombinant Human MIP-3α is an 8.0kDa protein containing 70 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

 名称缩写/Abbreviation：

rHuMIP-3α/CCL20

货号/Item No.：

UN183

别名/Synonyms：

LARC, Beta-chemokine Exodus-1, Liver and Activation-regulated Chemokine

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 8.0 kDa, a single non-glycosylated polypeptide chain containing 70 amino acids.

SCI的检索号/Accession #：

P78556 Ala27-Met96

蛋白序列/A.A. Sequence：

ASNFDCCLGYTDRILHPKFIVGFTRQLANEGCDINAIIFHTKKKLSVCANPKQTWVKYIVRLLSKKVKNM

纯度/Purity：

＞97% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 10-50 ng/ml.

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.1 EU/μg of rHuMIP-3α/CCL20 protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.