Recombinant Human Interleukin-8,77a.a./CXCL8

称：Recombinant Human Interleukin-8,77a.a./CXCL8

货号：UN080

规格：5μg/25μg/50μg/100μg/500μg/1mg

纯度：>96%

系统：E.coli.

产品详情

Recombinant Human Interleukin-8, 77a.a./CXCL8

Interleukin-8 (IL-8) is encoded by the IL8 gene and produced by macrophages and other cell types such as epithelial cells. It is also synthesized by endothelial cells, which store IL-8 in their storage vesicles. There are many receptors capable to bind IL-8, the most affinity to IL-8 are receptors CXCR1, and CXCR2. As a member of the CXC chemokine family, function of IL-8 is the induction of chemotaxis in its target cells, like neutrophil granulocytes, basophils, and T-cells. IL-8 is often associated with inflammation, it has been cited as a proinflammatory mediator in gingivitis and psoriasis. Mature human IL-8/CXCL8 shares 65%-69% amino acid sequence identity with canine, feline, and porcine IL-8/CXCL8. Recombinant Human IL-8 (CXCL8) (77a.a.) is an 8.9kDa protein containing   77 amino acid.

名称缩写/Abbreviation：  

rHuIL-8, 77a.a./CXCL8

货号/Item No.：  

UN080

别名/Synonyms：  

Emoctakin, GCP-1, MDNCF, MONAP, NAP-1, T-cell chemotactic factor, Protein 3-10C

表达系统/Source：  

Expressed in E. coli.

分子量/Molecular Weight：  

Approximately 8.9 kDa, a single non-glycosylated polypeptide chain containing 77 amino acids.

SCI的检索号/Accession #：  

P10145 Ala23-Ser99

蛋白序列/A.A. Sequence：  

AVLPRSAKELRCQCIKTYSKPFHPKFIKELRVIESGPHCANTEIIVKLSDGRELCLDPKENWVQRVVEKFLKRAENS

纯度/Purity：  

＞96% by SDS-PAGE or HPLC.

活性/Biological Activity：  

Fully biologically active when compared to standard. The ED₅₀ as determined by a chemotaxis bioassay using human CXCR2 transfected mouse BaF3 cells is less than 2 ng/ml, corresponding to a specific activity of ≥ 5.0×10⁵ IU/mg

配方/Formulation：  

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, with 150 mM NaCl, pH 7.4.

外观/Appearance：  

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：  

< 0.01 EU/μg of rHuIL-8,77a.a./CXCL8 protein as determined by LAL method.

复溶/Reconstitution：  

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：  

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.