Recombinant Human Granulocyte Colony-stimulating Factor

名称：Recombinant Human Granulocyte Colony-stimulating Factor

货号：UN046

规格：2μg/10μg/50μg/100μg/500μg/1mg

纯度：>97%

系统：E.coli.

产品详情

 Recombinant Human Granulocyte Colony-stimulating Factor

Granulocyte colony stimulating factor (G-CSF) is a pleiotropic cytokine. It is mainly produced by monocytes and macrophages upon activation by endotoxin, TNF-α and IFN-γ. Besides, many other cell types can secret this protein after activation by LPS, IL-1 or TNF-α. Various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively. G-CSF is cytokine that acts in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. In addition, it may function in some adhesion or recognition events at the cell surface. Human G-CSF is 73% identical at the amino acid level to murine G-CSF and the two proteins show species cross-reactivity. Recombinant human G-CSF is an 18.7kDa polypeptide containing 174 amino acid residues.

 名称缩写/Abbreviation：

rHuG-CSF

货号/Item No.：

UN046

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 18.7 kDa, a single non-glycosylated polypeptide chain containing 174 amino acids.

SCI的检索号/Accession #：

P09919 Thr31-Pro207

蛋白序列/A.A. Sequence：

TPLGPASSLPQSFLLKCLEQVRKIQGDGAALQEKLCATYKLCHPEELVLLGHSLGIPWAPLSSCPSQALQLAGCLSQLHSGLFLYQGLLQALEGISPELGPTLDTLQLDVADFATTIWQQMEELGMAPALQPTQGAMPAFASAFQRRAGGVLVASHLQSFLEVSYRVLRHLAQP

纯度/Purity：

＞97% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine NFS-60 cells is less than 0.1 ng/ml, corresponding to a specific activity of ≥ 1.0×10⁷ IU/mg.

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in 10 mM sodium acetate buffer, containing 3% (v/v) Trehalose, pH 4.0.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.1 EU/μg of rHuG-CSF protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.