Recombinant Human Keratinocyte Growth Factor/Fibroblast Growth Factor 7

名称：Recombinant Human Keratinocyte Growth Factor/Fibroblast Growth Factor 7

货号：UN128

规格：2μg/10μg/100μg/1mg

纯度：>96%

系统：E.coli.

产品详情

Recombinant Human Keratinocyte Growth Factor/ Fibroblast Growth Factor 7

KGF (also known as FGF-7) is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth, and the regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. KGF/FGF-7 is a mitogen factor specific for epithelial cells and keratinocytes. KGF/FGF-7 signals through FGFR 2b. KGF/FGF-7 plays a role in kidney and lung development, as well as in angiogenesis and wound healing. KGF actives on keratinocytes, and exhibits mitogenic activity for epidermal cells, but essentially no activity for fibroblasts. KGF has species cross active, human KGF shares 96% and 92% amino acid sequence identity with murine and rat FGF-7, respectively. Recombinant Human KGF/FGF-7 is an 18.9kDa protein consisting of 163 amino acid.

 名称缩写/Abbreviation：

KGF/FGF-7

货号/Item No.：

UN128

别名/Synonyms：

Keratinocyte Growth Factor, Fibroblast Growth Factor-7, HBGF-7

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 18.9 kDa, a single non-glycosylated polypeptide chain containing 163 amino acids.

SCI的检索号/Accession #：

P21781 Cys32-Thr194

蛋白序列/A.A. Sequence：

CNDMTPEQMATNVNCSSPERHTRSYDYMEGGDIRVRRLFCRTQWYLRIDKRGKVKGTQEMKNNYNIMEIRTVAVGIVAIKGVESEFYLAMNKEGKLYAKKECNEDCNFKELILENHYNTYASAKWTHNGGEMFVALNQKGIPVRGKKTKKEQKTAHFLPMAIT

纯度/Purity：

＞96% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. The ED₅₀ as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 10 ng/ml, corresponding to a specific activity of ≥ 1.0×10⁵ IU/mg.

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, with 500 mM NaCl, pH 8.0.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.01 EU/μg of rHuKGF/FGF-7 protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.