Recombinant Human 3-Hydroxy-3-methylglutaryl-CoA Reductase,CatalyticDomain

名称：Recombinant Human 3-Hydroxy-3-methylglutaryl-CoA Reductase,CatalyticDomain

货号：UN097

规格：100μg/1mg/10mg

纯度：>95%

系统：E.coli.

产品详情

Recombinant Human 3-Hydroxy-3-methylglutaryl-CoA Reductase, Catalytic Domain

3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGRC) Catalyzes the conversion of (3S) -hydroxy-3-methylglutaryl-CoA (HMG-CoA) to mevalonic acid, the rate-limiting step in the synthesis of cholesterol and other isoprenoids, thus plays a critical role in cellular cholesterol homeostasis. HMGCR is the main target of statins, a class of cholesterol-lowering drugs. Inhibition of HMGCR in the liver stimulates the LDL-receptors, which results in an increased clearance of LDL from the bloodstream and a decrease in blood cholesterol levels, The first result can be seen after one week of statin use and the effect is maximal after four to six weeks. Recombinant human HMGR is a 60.4kDa protein containing 560 amino acid.

 名称缩写/Abbreviation：

rHuHMGR

货号/Item No.：

UN097

别名/Synonyms：

HMG-CoA reductase

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 60.4 kDa, a single non-glycosylated polypeptide chain containing 560 amino acids.

SCI的检索号/Accession #：

P04035 Glu340-Ala888 with a C-terminal flag tag

蛋白序列/A.A. Sequence：

EQTETESTLSLKNPITSPVVTQKKVPDNCCRREPMLVRNNQKCDSVEEETGINRERKVEVIKPLVAETDTPNRATFVVGNSSLLDTSSVLVTQEPEIELPREPRPNEECLQILGNAEKGAKFLSDAEIIQLVNAKHIPAYKLETLMETHERGVSIRRQLLSKKLSEPSSLQYLPYRDYNYSLVMGACCENVIGYMPIPVGVAGPLCLDEKEFQVPMATTEGCLVASTNRGCRAIGLGGGASSRVLADGMTRGPVVRLPRACDSAEVKAWLETSEGFAVIKEAFDSTSRFARLQKLHTSIAGRNLYIRFQSRSGDAMGMNMISKGTEKALSKLHEYFPEMQILAVSGNYCTDKKPAAINWIEGRGKSVVCEAVIPAKVVREVLKTTTEAMIEVNINKNLVGSAMAGSIGGYNAHAANIVTAIYIACGQDAAQNVGSSNCITLMEASGPTNEDLYISCTMPSIEIGTVGGGTNLLPQQACLQMLGVQGACKDNPGENARQLARIVCGTVMAGELSLMAALAAGHLVKSHMIHNRSKINLQDLQGACTKKTAGGGSDYKDDDK

纯度/Purity：

＞95% by SDS-PAGE under reducing conditions.

配方/Formulation：

A 0.2 μm filtered concentrated solution in 10 mM PB, with 50% (v/v) Glycerol, 2.5 mM DTT, pH 7.4.

外观/Appearance：

Sterile colorless liquid.

浓度/Concentration：

No less than 10mg/ml, The concentration for a specific batch as shown in the COA

内毒素/Endotoxin：

< 0.5 EU/μg of rHuHMGR protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile aqueous buffer to an appropriate concentration. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 12 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.