Recombinant Human Fibroblast Growth Factor-9

名称：Recombinant Human Fibroblast Growth Factor-9

货号：UN136

规格：5μg/25μg/50μg/100μg/1mg

纯度：>96%

系统：E.coli.

产品详情

Recombinant Human Fibroblast Growth Factor-9

Fibroblast growth factor-9 (FGF-9) is a member of the fibroblast growth factor (FGF) family. All FGF family members are heparin binding growth factors with a core 120 amino acids FGF domain that allows for a common tertiary structure. FGF-9 plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. FGF-9 is a monomer and interacts with FGFR1, FGFR2, FGFR3 and FGFR4. The human FGF-9 shares 98% a.a. sequence identity with mouse, rat, equine, porcine, and bovine FGF-9. Recombinant Human FGF-9 is a 23.3kDa protein consisting of 207 amino acid residues.

 名称缩写/Abbreviation：

rHuFGF-9

货号/Item No.：

UN136

别名/Synonyms：

Fibroblast Growth Factor-9, GAF (Glia-activating factor), HBGF-9

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 23.3 kDa, a single non-glycosylated polypeptide chain containing 207 amino acids.

SCI的检索号/Accession #：

P31371 Ala2-Ser208

蛋白序列/A.A. Sequence：

APLGEVGNYFGVQDAVPFGNVPVLPVDSPVLLSDHLGQSEAGGLPRGPAVTDLDHLKGILRRRQLYCRTGFHLEIFPNGTIQGTRKDHSRFGILEFISIAVGLVSIRGVDSGLYLGMNEKGELYGSEKLTQECVFREQFEENWYNTYSSNLYKHVDTGRRYYVALNKDGTPREGTRTKRHQKFTHFLPRPVDPDKVPELYKDILSQS

纯度/Purity：

＞96% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. The ED₅₀ as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of ≥ 2.0×10⁶ IU/mg

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in PBS pH 7.4.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.01 EU/μg of rHuFGF-9 protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.