Recombinant Human Insulin

名称：Recombinant Human Insulin

货号：UN416

规格：5mg/20mg/100mg/1g/5g

纯度：>96%

系统：E.coli.

产品详情

Recombinant Human Insulin

Insulin is a double-chain polypeptide hormone secreted by β-cells of pancreatic islets. The α chain and β chain are connected by two interchain disulfide bonds, while the α chain contains an intra-chain disulfide bond. Insulin can activate downstream signaling pathways by binding to insulin receptors, including insulin receptor phosphorylation, IRS-1 phosphorylation, Akt phosphorylation, GSK3β phosphorylation, etc., to promote the uptake, utilization and storage of glucose, amino acids. and fatty acids by cells. Inhibits the breakdown of glycogen, protein or fat. Insulin is a common addition to serum-free media. Human insulin can be used in cultured cells of mice, rats and other mammals, and can also be directly injected into mice or rats for related experimental studies. Recombinant human insulin is a 5.8kDa protein heterodimeric glycoprotein consisting of disulfide-linked B chain and A chain (51 total amino acid) .

 名称缩写/Abbreviation：

rHuinsulin

货号/Item No.：

UN416

别名/Synonyms：

INS

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 5.8 kDa, a single non-glycosylated polypeptide chain containing 51 amino acids.

SCI的检索号/Accession #：

P01308 B chain: Phe25-Thr54, A chain: Gly90-Asn110

蛋白序列/A.A. Sequence：

B chain: FVNQHLCGSHLVEALYLVCGERGFFYTPKT A chain: GIVEQCCTSICSLYQLENYCN

纯度/Purity：

＞96% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to insulin standard. The potency is determined by its ability to reduce blood glucose levels in healthy adult mice under a twin cross-over design. The expected potency is ≥ 20 IU/mg.

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in 10 mM HCl.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.01 EU/μg of rHuinsulin protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10 mM HCl to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.