Recombinant Human Fibroblast Growth Factor-4

名称：Recombinant Human Fibroblast Growth Factor-4

货号：UN131

规格：5μg/25μg/50μg/100μg/500μg/1mg

纯度：>96%

系统：E.coli.

产品详情

Recombinant Human Fibroblast Growth Factor-4

Fibroblast Growth Factor 4 (FGF-4, also named FGFK and KFGF) belongs to the large FGF family which has at least 23 members. FGF-4 has functions that maintain a population of progenitor cells in the epiblast that generates mesoderm, and contribute to the stem cell population that is incorporated in the tailbud. It is also required for axial elongation of the mouse embryo after gastrulation. Mature human FGF4 shares 91%, 82%, 94% and 91% a.a. identity with murine, rat, canine and bovine FGF4. Additionally, FGF-4 shares about 30% sequence identity with the prototypical members of the FGF family. Recombinant human FGF-4 is a 19.8kDa protein containing 182 amino-acid residues.

 名称缩写/Abbreviation：

rHuFGF-4

货号/Item No.：

UN131

别名/Synonyms：

Fibroblast Growth Factor-4, HST-1, Transforming protein KS3, HBGF-4

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 19.8 kDa, a single non-glycosylated polypeptide chain containing 182 amino acids.

SCI的检索号/Accession #：

P08620 Gly25-Leu206

蛋白序列/A.A. Sequence：

GRGGAAAPTAPNGTLEAELERRWESLVALSLARLPVAAQPKEAAVQSGAGDYLLGIKRLRRLYCNVGIGFHLQALPDGRIGGAHADTRDSLLELSPVERGVVSIFGVASRFFVAMSSKGKLYGSPFFTDECTFKEILLPNNYNAYESYKYPGMFIALSKNGKTKKGNRVSPTMKVTHFLPRL

纯度/Purity：

＞96% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. The ED₅₀ as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of ≥ 2.0×10⁶ IU/mg

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, with 500 mM NaCl, 5% (v/v) Trehalose and 0.02% (v/v) Tween-20, pH 7.4.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.1 EU/μg of rHuFGF-4 protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.