Recombinant Human Interleukin-4

名称：Recombinant Human Interleukin-4

货号：UN030

规格：5μg/20μg/50μg/100μg/500μg/1mg

纯度：>98%

系统：E.coli.

产品详情

Recombinant Human Interleukin-4

Interleukin-4 (IL-4) is a pleiotropic cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells and produced primarily by mast cells, Th2 cells, eosinophils and basophils. IL-4 is a key regulator in humoral and adaptive immunity and has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of B cells into plasma cells. It regulates the differentiation of naive CD4+ T cells into helper Th2 cells and immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells is associated with allergies. Mature human IL-4 shares 55%, 39% and 43% aa sequence identity with bovine, mouse, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities. IL-4 has a compact, globular fold, stabilized by 3 disulfide bonds. The recombinant Interleukin-4 is a 15kDa protein consisting of 129amino acid.

 名称缩写/Abbreviation：

rHuIL-4

货号/Item No.：

UN030

别名/Synonyms：

BCGF, BCDF, B cell stimulating factor (BSF-1)

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 15.0 kDa, 129 amino acids.

SCI的检索号/Accession #：

P05112 His25-Ser153

蛋白序列/A.A. Sequence：

HKCDITLQEIIKTLNSLTEQKTLCTELTVTDIFAASKNTTEKETFCRAATVLRQFYSHHEKDTRCLGATAQQFHRHKQLIRFLKRLDRNLWGLAGLNSCPVKEANQSTLENFLERLKTIMREKYSKCSS

纯度/Purity：

＞98% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using human TF-1 cells is less than 0.2 ng/ml, corresponding to a specific activity of ≥ 5.0×10⁶ IU/mg.

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, with 150 mM NaCl, 0.02% (v/v) Tween-20, pH 5.0.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.1 EU/μg of rHuIL-4 protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.