Recombinant Human Interleukin-21

名称：Recombinant Human Interleukin-21

货号：UN089

规格：2μg/10μg/50μg/100μg/1mg

纯度：>97%

系统：E.coli.

产品详情

Recombinant Human Interleukin-21

Human IL-21 is encoded by IL-21 gene located on Chr.4. It is a pleiotropic cytokine produced by CD4+ T cells in response to antigenic stimulation and can regulating immune system cells, for instance cytotoxin T cells and natural killer cells. Additionally, it can induce target cells division or proliferation. IL-21 elicits its effect through binding to IL-21R, which also contains the gamma chain found in other cytokine receptors such as IL-2, IL-4, IL-7, IL-9 and IL-15. IL-21 shows having much relation with clinical illnesses, including cancer immunotherapy, viral infections and allergies. Mature human IL-21 protein shares 63% and 61% aa sequence identity with mouse and rat IL-21 protein, respectively. Recombinant IL-21 is a single non-glycosylated polypeptide chain containing 133 amino acids

 名称缩写/Abbreviation：

rHuIL-21

货号/Item No.：

UN089

别名/Synonyms：

Interleukin-21, Za11

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 15.4 kDa, a single non-glycosylated polypeptide chain containing 133 amino acid.

SCI的检索号/Accession #：

Q9HBE4 Gln30-Ser162

蛋白序列/A.A. Sequence：

QGQDRHMIRMRQLIDIVDQLKNYVNDLVPEFLPAPEDVETNCEWSAFSCFQKAQLKSANTGNNERIINVSIKKLKRKPPSTNAGRRQKHRLTCPSCDSYEKKPPKEFLERFKSLLQKMIHQHLSSRTHGSEDS

纯度/Purity：

＞97% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED₅₀ for this effect is ≤ 2 ng/ml. The specific activity of recombinant human IL-21 ≥ 5.0×10⁵ U/mg.

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, with 150 mM NaCl, pH 7.4.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.01 EU/μg of rHuIL-21 protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.