Recomb inant Human Interleukin-11

名称：Recomb inant Human Interleukin-11

货号：UN082

规格：2μg/10μg/50μg/100μg/1mg

纯度：>96%

系统：E.coli.

产品详情

Recombinant Human Interleukin-11

IL-11 is a multifunctional cytokine produced by stromal cells, such as fibroblasts, epithelial cells and osteoclasts. IL-11 plays an important regulatory role in hematopoiesis by stimulating growth of myeloid, erythroid and megakaryocyte progenitor cells. It also regulates bone metabolism, inhibits production of proinflammatory cytokines, and protects against gastromucosal injury The functions of IL-11 are that it directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells, and induces megakaryocyte maturation resulting in increased platelet production. Mature human IL-11 shares 88%, 88%, and 96% aa sequence identity with mouse, rat and canine IL-11, respectively. Recombinant Human IL-11 is a 19.1kDa globular protein containing 179 amino acid.

 名称缩写/Abbreviation：

rHuIL-11

货号/Item No.：

UN082

别名/Synonyms：

AGIF (Adipogenesis inhibitory factor)

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 19.1 kDa, a single non-glycosylated polypeptide chain containing 179 amino acids.

SCI的检索号/Accession #：

P20809 Pro22-Leu199 with an N-terminal Met

蛋白序列/A.A. Sequence：

MPGPPPGPPRVSPDPRAELDSTVLLTRSLLADTRQLAAQLRDKFPADGDHNLDSLPTLAMSAGALGALQLPGVLTRLRADLLSYLRHVQWLRRAGGSSLKTLEPELGTLQARLDRLLRRLQLLMSRLALPQPPPDPPAPPLAPPSSAWGGIRAAHAILGGLHLTLDWAVRGLLLLKTRL

纯度/Purity：

＞96% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. The ED₅₀ was determined by the dose-dependent stimulation of the proliferation of murine T11 cells is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5×10⁵ units/mg.

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, with 150 mM NaCl, 150 mM Glycine, 5% (v/v) Trehalose, pH 7.2.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.01 EU/μg of rHuIL-11 protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.