Recombinant Human Interferon-gamma

名称：Recombinant Human Interferon-gamma

货号：UN004

规格：5μg/20μg/50μg/100μg/500μg/1mg

纯度：>98%

系统：E.coli.

产品详情

Recombinant Human Interferon-gamma

Interferon-gamma (IFN-γ) , also known as Type II interferon or immune interferon, is a dimerized soluble cytokine that is critical for innate and adaptive immunity against viral, some bacterial and protozoal infections. It is produced primarily by CD4 and CD8 T lymphocytes as well as natural killer (NK) and natural killer T (NKT) cells. The primary role for IFN-γ is the activation of macrophages to increase phagocytosis, tumoricidal properties, and intracellular killing of pathogens. IFN-γ also increases expression of MHC antigens by macrophages and this facilitates antigen presentation to T cells. The recombinant Interferon-gamma s a 16.9kDa protein consisting of 144 amino acid.

 名称缩写/Abbreviation：

rHuIFN-γ

货号/Item No.：

UN004

别名/Synonyms：

Immune Interferon, type II interferon, T cell interferon, MAF

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 16.9 kDa, a single non-glycosylated polypeptide chain containing 144 amino acids.

SCI的检索号/Accession #：

P01579 Gln2-Gln166 with an N-terminal Met

蛋白序列/A.A. Sequence：

MQDPYVKEAENLKKYFNAGHSDVADNGTLFLGILKNWKEESDRKIMQSQIVSFYFKLFKNFKDDQSIQKSVETIKEDMNVKFFNSNKKKRDDFEKLTNYSVTDLNVQRKAIHELIQVMAELSPAAKTGKRKRSQMLFRGRRASQ

纯度/Purity：

＞98% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. The ED₅₀ as measured in anti-viral assays using human HeLa cells infected with encephalomyocarditis (EMC) virus is 0.15~0.80 ng/ml.

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, with 5% (v/v) Trehalose, pH 7.4.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.01 EU/μg of rHuIFN-γ protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.