Recombinant Human Fibronectin Fragment

名称：Recombinant Human Fibronectin Fragment

货号：UN011

规格：500μg/1mg/2.5mg

纯度：>97%

系统：E.coli.

产品详情

Recombinant Human Fibronectin Fragment

Fibronectin (FN) is a large, modular glycoprotein that generates a polymeric fibrillar network in the extracellular matrix (ECM) , and forms soluble, disulfide-linked dimeric protomers in plasma and other body fluids. Fibronectin is a ligand for many molecules, including fibrin, heparin, chondroitin sulfate, collagen/gelatin, and integrin. It is involved in multiple cellular processes such as cell adhesion/migration, blood clotting, morphogenesis, tissue repair, and cell signaling.

Recombinant Human Fibronectin fragment is a recombinant fibronectin fragment composed of three functional domains: the cell-binding domain (C-domain) , heparin-binding domain (H-domain) , and CS-1 sequence. The fragment can improve the efficiency of retroviral-mediated gene transduction by helping the co-localization of target cells and virions

 名称缩写/Abbreviation：

rHuFibronectin Fragment

货号/Item No.：

UN011

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 62.6 kDa, a single non-glycosylated polypeptide chain containing 574 amino acids

纯度/Purity：

＞97% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. Measured by its ability to support cell attachment and spreading when used as a substratum for cell culture. In this application, the recommended concentration for this effect is typically 1-5 μg/cm^2. Fibronectin can also be added to the media to support cell spreading at a concentration of 0.5-50 μg/ml.

配方/Formulation：

A 0.2 μm filtered concentrated solution in 1 × PBS, with 5% (v/v) Trehalose, 0.02% (v/v) Tween-20, pH 7.4.

外观/Appearance：

Sterile colorless liquid.

浓度/Concentration：

No less than 10mg/ml, The concentration for a specific batch as shown in the COA

内毒素/Endotoxin：

< 0.01 EU/μg of rFN-CH-296 protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile aqueous buffer to an appropriate concentration. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 12 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.