Recombinant Human 4-1BB Receptor

名称：Recombinant Human 4-1BB Receptor

货号：UN404

规格：5μg/20μg/100μg/500μg/1mg

纯度：>97%

系统：E.col

产品详情

Recombinant Human 4-1BB Receptor

4-1BB receptor, also named TNFRSF9 is a member of the TNF superfamily of receptors. It is mainly expressed on the surface of a variety of T cells, but also found in B cells, monocytes, and various transformed cell lines. 4-1BB receptor binds to 4-1BBL, and they co-stimulate activity for activated T cells. Signaling by 4-1BB Receptor has been implicated in the antigen-presentation process and generation of cytotoxic T cells. Crosslinking of 4-1BB Receptor enhances T cell proliferation, IL-2 secretion survival and cytolytic activity. Further, it can enhance immune activity to eliminate tumors in mice. The human 4-1BB Receptor gene codes for a 255 amino acid type I transmembrane protein containing a 17 amino acid N-terminal signal sequence, a 169 amino acid extracellular domain, a 27 amino acid transmembrane domain and a 42 amino acid cytoplasmic domain. Recombinant Human soluble 4-1BB Receptor is a 166 amino acid polypeptide, which contains the cysteine-rich TNFR-like extracellular domain of 4-1BB Receptor.

名称缩写/Abbreviation：

rHu4-1BB Receptor

货号/Item No.：

UN404

别名/Synonyms：

TNFRSF9, CD137, T-cell antigen ILA, CDw137

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 17.7 kDa, a single non-glycosylated polypeptide chain containing 166 amino acids.

SCI的检索号/Accession #：

Q07011 Glu19-Ser184

蛋白序列/A.A. Sequence：

ERTRSLQDPCSNCPAGTFCDNNRNQICSPCPPNSFSSAGGQRTCDICRQCKGVFRTRKECSSTSNAECDCTPGFHCLGAGCSMCEQDCKQGQELTKKGCKDCCFGTFNDQKRGICRPWTNCSLDGKSVLVNGTKERDVVCGPSPADLSPGASSVTPPAPAREPGHS

纯度/Purity：

＞97% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. The biological activity is determined by its inhibitory effect of IL-8 production using human peripheral blood mononuclear cells. About 90 % of inhibition was seen using a concentration of 1 μg for both 4-1BB Ligand and 4-1BB Receptor.

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, with 150 mM NaCl, pH 5.8.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.1 EU/μg of rHu4-1BB Receptor protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.