Recomb inant Murine Sonic Hedgehog

名称：Recomb inant Murine Sonic Hedgehog

货号：UN303

规格：5μg/25μg/100μg/500μg/1mg

纯度：>96%

系统：E.coli.

产品详情

Recombinant Murine Sonic Hedgehog

Sonic hedgehog is one of three proteins in the mammalian signaling pathway family called hedgehog, the others being desert hedgehog (DHH) and Indian hedgehog (IHH) . As the hh homologues, SHH has been found to have the most critical roles in development. It acts as a morphogen involved in patterning many systems, including the limb and midline structures in the brain, spinal cord, the thalamus by the zona limitans intrathalamica and the teeth. There SHH undergoes auto-processing to generate a 20kDa N-terminal signaling domain (SHH-N) and a 25kDa C-terminal domain with no known signaling role. Recombinant Murine Sonic Hedgehog is a 20.0kDa protein consisting of 176 amino acid residues, including an N-terminal Ile-Val-Ile sequence substituted for the naturally occurring, chemically modified Cys residue.

 名称缩写/Abbreviation：

rMuSHH

货号/Item No.：

UN303

别名/Synonyms：

Hhg-1

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 19.8 kDa, a single non-glycosylated polypeptide chain containing 176 amino acids.

SCI的检索号/Accession #：

Q62226 Cys25-Gly198 (C25IVI)

蛋白序列/A.A. Sequence：

IVIGPGRGFGKRRHPKKLTPLAYKQFIPNVAEKTLGASGRYEGKITRNSERFKELTPNYNPDIIFKDEENTGADRLMTQRCKDKLNALAISVMNQWPGVKLRVTEGWDEDGHHSEESLHYEGRAVDITTSDRDRSKYGMLARLAVEAGFDWVYYESKAHIHCSVKAENSVAAKSGG

纯度/Purity：

＞96% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. The ED₅₀ as determined by inducing alkaline phosphatase production of murine C3H/10T1/2 cells is 0.5 - 1.0 μg/ml.

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, with 150 mM NaCl, pH 7.4.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.1 EU/μg of rMuSHH protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.