Recombinant Murine R-Spondin 1

名称：Recombinant Murine R-Spondin 1

货号：UN073

规格：5μg/10μg/50μg/100μg/250μg/1mg

纯度：>96%

系统：E.coli.

产品详情

Recombinant Murine R-Spondin 1

R-Spondin 1, also known as cysteine-rich and single thrombospondin domain containing protein 3 (Cristin 3) , is a 27kDa secreted protein that shares ~40% amino acid (aa) identity with three other R-Spondin family members (1, 2) . All R-Spondins regulate Wnt/beta-Catenin signaling but have distinct expression patterns. R-Spondin 1 competes with the Wnt antagonist DKK-1 for binding to the Wnt co-receptors, Kremen and LRP-6, reducing their DKK-1-mediated internalization. However, reports are mixed on whether R-Spondin 1 binds LRP-6 directly. R-Spondin 1 is expressed in early development at the roof plate boundary and is thought to contribute to dorsal neural tube development. I Postnatally, R-Spondin 1 is expressed by neuroendocrine cells in the intestine, adrenal gland and pancreas, and by epithelia in kidney and prostate (9) . Injection of recombinant R-Spondin 1 in mice causes activation of beta-catenin and proliferation of intestinal crypt epithelial cells, and ameliorates experimental colitis. Mouse R-Spondin 1 shares 98%, 94%, 94%, 93%, 92% and 88% aa identity with rat, human, horse, cow, goat and dog RSPO-1, respectively, within aa 21-209. Recombinant Mouse R-Spondin 1 is a single non-glycosylated polypeptide chain containing 189 amino acids

 名称缩写/Abbreviation：

rMuR-spondin 1

货号/Item No.：

UN073

别名/Synonyms：

Cristin3; R-Spondin 1; R-spondin1; R-spondin-1

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 20.9 kDa, a single non-glycosylated polypeptide chain containing 189 amino acids.

SCI的检索号/Accession #：

Q9Z132 Ser21-Gly209

蛋白序列/A.A. Sequence：

SRGIKGKRQRRISAEGSQACAKGCELCSEVNGCLKCSPKLFILLERNDIRQVGVCLPSCPPGYFDARNPDMNKCIKCKIEHCEACFSHNFCTKCQEALYLHKGRCYPACPEGSTAANSTMECGSPAQCEMSEWSPWGPCSKKRKLCGFRKGSEERTRRVLHAPGGDHTTCSDTKETRKCTVRRTPCPEG

纯度/Purity：

＞96% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The typical ED₅₀ is 50-200 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt-3a.

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, with 150 mM NaCl, pH 7.4.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.01 EU/μg of rMuR-spondin 1 protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.