Recombinant Murine Oncostatin-M

名称：Recombinant Murine Oncostatin-M

货号：UN518

规格：2μg/10μg/50μg/100μg/500μg/1mg

纯度：>96%

系统：E.coli.

产品详情

Recombinant Murine Oncostatin-M

Oncostatin-M (OSM) is a multifunctional cytokine that belongs to the Interleukin-6 subfamily. Among the family members, OSM is most closely related to leukemia inhibitory factor (LIF) and it in fact utilizes the LIF receptor in addition to its specific receptor in the human. A biologically active OSM receptor has been previously described that consists of a heterodimer of leukemia inhibitory factor receptor (LIFR) and gp130. OSM is synthesized by stimulated T-cells and monocytes. Furthermore, the effects of OSM on endothelial cells suggest a pro-inflammatory role for OSM and endothelial cells possess a large number of OSM receptors. Recombinant murine OSM contains 181 amino acids and has a molecular mass of 20.4 kDa. It has approximately 48 % and 72 % amino acid sequence identity with human and rat OSM.

 名称缩写/Abbreviation：

rMuOSM

货号/Item No.：

UN518

别名/Synonyms：

Oncostatin M; Oncostatin-M; OSM

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 20.4 kDa, a single non-glycosylated polypeptide chain containing 181 amino acids.

SCI的检索号/Accession #：

P53347 Asn1-Arg181

蛋白序列/A.A. Sequence：

NRGCSNSSSQLLSQLQNQANLTGNTESLLEPYIRLQNLNTPDLRAACTQHSVAFPSEDTLRQLSKPHFLSTVYTTLDRVLYQLDALRQKFLKTPAFPKLDSARHNILGIRNNVFCMARLLNHSLEIPEPTQTDSGASRSTTTPDVFNTKIGSCGFLWGYHRFMGSVGRVFREWDDGSTRSR

纯度/Purity：

＞96% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine NIH-3T3 cells is less than 2.0 ng/ml, corresponding to a specific activity of ≥ 5.0×10⁵ IU/mg.

配方/Formulation：

A 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4, with 5 % trehalose.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

浓度/Concentration：

No less than 10mg/ml, The concentration for a specific batch as shown in the COA

内毒素/Endotoxin：

< 0.1 EU/μg of rMuOSM protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile aqueous buffer to an appropriate concentration. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 12 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.