Recomb inant Murine Fibroblast Growth Factor 4

名称：Recomb inant Murine Fibroblast Growth Factor 4

货号：UN491

规格：5μg/25μg/50μg/100μg/250μg/500μg/1mg

纯度：>96%

系统：E.coli.

产品详情

Recombinant Murine Fibroblast Growth Factor 4

FGF-4 is a 25kDa secreted, heparin-binding member of the FGF family. The mouse FGF-4 cDNA encodes 202 amino acids (aa) with a 29 aa signal sequence and a 173 aa mature protein with an FGF homology domain that contains a heparin binding region near the C-terminus. Mature mouse FGF-4 shares 87% and 90% aa identity with human and rat FGF-4, respectively. Human FGF-4 has been shown to exhibit cross species activity. Its expression in the trophoblast inner cell mass promotes expression of FGF R2, and is required for maintenance of the trophectoderm and primitive endoderm. Later in development, FGF-4 works together with FGF-8 to mediate the activities of the apical ectodermal ridge, which direct the outgrowth and patterning of vertebrate limbs. FGF-4 is proposed to play a physiologically relevant role in human embryonic stem cell self-renewal. It promotes stem cell proliferation, but may also aid differentiation depending on context and concentration, and is often included in embryonic stem cell media in vitro. A C-terminally truncated 15kDa isoform that opposes full-length FGF-4 and promotes differentiation is endogenously expressed in human embryonic stem cells. FGF-4 is mitogenic for fibroblasts and endothelial cells in vitro and has autocrine transforming potential. It is a potent angiogenesis promoter in vivo and has been investigated as therapy for coronary artery disease. Recombinant murine FGF-4 is a 19kDa globular protein containing 173 amino acid residues.

 名称缩写/Abbreviation：

rMuFGF-4

货号/Item No.：

UN491

别名/Synonyms：

FGFK; KFGF; HBGF-4

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 19.0 kDa, a single non-glycosylated polypeptide chain containing 173 amino acids.

SCI的检索号/Accession #：

P11403 Ala30-Leu202

蛋白序列/A.A. Sequence：

APNGTRHAELGHGWDGLVARSLARLPVAAQPPQAAVRSGAGDYLLGLKRLRRLYCNVGIGFHLQVLPDGRIGGVHADTRDSLLELSPVQRGVVSIFGVASRFFVAMSSRGKLFGVPFFTDECKFKEILLPNNYNAYEAYAYPGMFMALSKNGRTKKGNRVSPTMKVTHFLPRL

纯度/Purity：

＞96% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. Measured in a cell proliferation assay using NR6R3T3 mouse fibroblasts, the ED₅₀ for this effect is typically 0.75-3.75 ng/ml.

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, with 500 mM NaCl, 5% (v/v) Trehalose 0.02% (v/v) Tween-20, pH 7.4.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.01 EU/μg of rMuFGF-4 protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled PBS or aqueous buffer containing 0.1% BSA to a concentration of 0.1-0.5 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.