Recombinant Human/Murine Fibroblast Growth Factor-8b

名称：Recombinant Human/Murine Fibroblast Growth Factor-8b

货号：UN135

规格：5μg/25μg/50μg/100μg/1mg

纯度：>97%

系统：E.coli.

产品详情

Recombinant Human/Murine Fibroblast Growth Factor-8b

FGF-8 (FGF-8b) is a heparin-binding growth factor belonging to the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. There are 4 known alternate spliced forms of FGF8 (FGF-8A, 8B, 8E and 8F) . The human and murine FGF-8A and B are identical, unlike human and mouse FGF-8E and F, which are 98% identical. FGF-8 targets mammary carcinoma cells and other cells expressing the FGF receptors. Recombinant Human/Murine FGF-8 (FGF-8b) is a 22.5kDa protein consisting of 194 amino acid residues.

 名称缩写/Abbreviation：

rHu/MuFGF-8b

货号/Item No.：

UN135

别名/Synonyms：

Fibroblast Growth Factor-8, FGF-8b, AIGF, HBGF-8

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 22.5 kDa, a single non-glycosylated polypeptide chain containing 194 amino acids.

SCI的检索号/Accession #：

P55075-3 Gln23-Arg215 with an N-terminal Met

蛋白序列/A.A. Sequence：

MQVTVQSSPNFTQHVREQSLVTDQLSRRLIRTYQLYSRTSGKHVQVLANKRINAMAEDGDPFAKLIVETDTFGSRVRVRGAETGLYICMNKKGKLIAKSNGKGKDCVFTEIVLENNYTALQNAKYEGWYMAFTRKGRPRKGSKTRQHQREVHFMKRLPRGHHTTEQSLRFEFLNYPPFTRSLRGSQRTWAPEPR

纯度/Purity：

＞97% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using Balb/c 3T3 cells. The expected ED₅₀ is 2.0-4.0 ng/ml, corresponding to a specific activity of ≥ 2.5×10⁵ IU/mg

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, with 500 mM NaCl, 5% (v/v) Trehalose and 0.02% (v/v) Tween-20, pH 7.4.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.01 EU/μg of rHu/MuFGF-8b protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.