Recombinant Human Transforming Growth Factor alpha

名称：Recombinant Human Transforming Growth Factor alpha

货号：UN426

规格：5μg/20μg/50μg/100μg/500μg/1mg

纯度：>96%

系统：E.coli.

产品详情

Recombinant Human Transforming Growth Factor alpha

TGF-α is an EGF-related polypeptide growth factor that signals through the EGF receptor, and stimulates the proliferation of a wide range of epidermal and epithelial cells. It is produced by monocytes, keratinocytes, and various tumor cells. TGF-α induces anchorage-independence transformation in cultured cells. TGF-alpha binds to the EGF receptor and activates the receptor tyrosine kinase. Accordingly, TGF-alpha shows a similar potency to EGF as a mitogen for fibroblasts and as an inducer of epithelial development in vivo. TGF-alpha is reportedly more potent than EGF as an angiogenic factor in vivo and as a stimulator for keratinocyte migration. The EGF receptor gene represents the cellular homologue of the avian v-erb-B oncogene Human, murine and rat TGF-α are cross-species reactive. Recombinant Human TGF-α is a 51 amino acid polypeptide (5.7kDa) which shares approximately 40% sequence homology with EGF, including 6 conserved cysteine residues, which form 3 intramolecular disulfide bonds.

 名称缩写/Abbreviation：

rHuTGFα

货号/Item No.：

UN426

别名/Synonyms：

Transforming Growth Factor-α, Sacroma growth factor, TGF-type I, ETGF

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 5.7 kDa, a single non-glycosylated polypeptide chain containing 51 amino acids.

SCI的检索号/Accession #：

P01135 Val40-Ala89 with an N-terminal Met

蛋白序列/A.A. Sequence：

MVVSHFNDCPDSHTQFCFHGTCRFLVQEDKPACVCHSGYVGARCEHADLLA

纯度/Purity：

＞96% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. The ED₅₀ as determined by the dose-dependent stimulation of thymidine uptake by BALB/c 3T3 cells is ≤ 0.2 ng/ml, corresponding to a specific activity of ≥ 5×10⁶ units/mg

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, with 0.02% (v/v) Tween-20, pH 7.4.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.01 EU/μg of rHuTGFα protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.