Recombinant Human Stromal-Cell Derived Factor-1 beta/CXCL12 alpha

名称：Recombinant Human Stromal-Cell Derived Factor-1 beta/CXCL12 alpha

货号：UN207

规格：2μg/10μg/50μg/100μg/250μg/500μg/1mg

纯度：>97%

系统：E.coli.

产品详情

Recombinant Human Stromal-Cell Derived Factor-1 beta/CXCL12 alpha

CXCL12 also known as SDF-1 is belonging to the CXC chemokine family. It is encoded by the CXCL12 gene. In recently study, Human CXCL12 is expressed as six isoforms that differ only in the C-terminal tail. And all SDF-1 isoforms undergo proteolytic processing of the first two N-terminal amino acids. In all SDF-1 isoforms, SDF-1β is the canonical sequence. Contrast to the canonical sequence SDF-1β, SDF-1α is shorter by four amino acids at the C-terminal tail. On the cell surface, the receptor for this chemokine is CXCR4 and syndecan 4. CXCL12 is strongly chemotactic for T-lymphocytes, monocytes, but not neutrophils. CXCL12 is a very important factor in carcinogenesis and the neovascularization linked to tumor progression. Recombinant human Stromal-Cell Derived Factor-1 alpha/CXCL12 alpha is an 8.0kDa protein containing 68 amino acid residues.

 名称缩写/Abbreviation：

rHuSDF-1α/CXCL12α

货号/Item No.：

UN207

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 8.0 kDa, a single non-glycosylated polypeptide chain containing 68 amino acids.

SCI的检索号/Accession #：

P48061 Lys22-Lys89

蛋白序列/A.A. Sequence：

KPVSLSYRCPCRFFESHVARANVKHLKILNTPNCALQIVARLKNNNRQVCIDPKLKWIQEYLEKALNK

纯度/Purity：

＞97% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using PHA and rHuIL-2 activated human peripheral blood T-lymphocytes is in a concentration range of 20-80 ng/ml.

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.01 EU/μg of rHuSDF-1α/CXCL12α protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.