Recombinant Human Soluble Tumor Necrosis Factor-RelatedApoptosis-inducing Ligand Receptor-2/TNFRSF10B

名称：Recombinant Human Soluble Tumor Necrosis Factor-RelatedApoptosis-inducing Ligand Receptor-2/TNFRSF10B

货号：UN405

规格：10μg/50μg/100μg/500μg/1mg

纯度：>97%

系统：E.coli.

产品详情

Recombinant Human Soluble Tumor Necrosis Factor-Related Apoptosis-inducing Ligand Receptor-2/TNFRSF10B

Tumor necrosis factor-related apoptosis-inducing ligand Receptor 2 (TRAIL-R2) is a cell-surface receptor involved in tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) -induced cell-death signaling. The death ligand TRAIL bears high potential as a new anticancer agent, as binding to the death receptors TRAIL-R1 or TRAIL-R2 triggers apoptosis in most cancer cells. TRAIL-R2 has been shown to be associated with a decrease in the survival rates of breast cancer patients. Recombinant Human soluble TRAILR2/TNFRSF10B is a 14.8kDa protein (132 amino acid) consisting of the TNFR-homologous, cysteine-rich portion of the extracellular domain.

 名称缩写/Abbreviation：

rHusTRAILR2/TNFRSF10B

货号/Item No.：

UN405

别名/Synonyms：

soluble TRAIL Receptor-2, DR5, TNFRSF10B, KILER, TRICK2A, TRICKB

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 14.8 kDa, a single non-glycosylated polypeptide chain containing 132 amino acids.

SCI的检索号/Accession #：

O14763 Glu52-Ser183

蛋白序列/A.A. Sequence：

ESALITQQDLAPQQRAAPQQKRSSPSEGLCPPGHHISEDGRDCISCKYGQDYSTHWNDLLFCLRCTRCDSGEVELSPCTTTRNTVCQCEEGTFREEDSPEMCRKCRTGCPRGMVKVGDCTPWSDIECVHKES

纯度/Purity：

＞97% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. rHusTRAIL-R2 reduced the production of LPS-induced TNF by its ability to neutralize endogenous TRAIL in fresh human PBMC. In this assay, endogenous TRAIL is induced during a 24-hour exposure to LPS (10 ng/mL) but in the presence of rHusTRAIL-R2, TRAIL-induced TNF is suppressed.

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, with 150 mM NaCl, pH 7.4.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.1 EU/μg of rHusTRAILR2/TNFRSF10B protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY