Recombinant Human Soluble Receptor Activator of Nuclear Factor kappa-BReceptor/TNFRSF11A

名称：Recombinant Human Soluble Receptor Activator of Nuclear Factor kappa-BReceptor/TNFRSF11A

货号：UN408

规格：20μg/100μg/250μg/500μg/1mg

纯度：>98%

系统：E.coli.

产品详情

Recombinant Human Soluble Receptor Activator of Nuclear Factor kappa-B Receptor/TNFRSF11A

Human soluble Receptor Activator of NF-κB Receptor RANK Ligand and RANK Receptor are members of the TNF superfamily that play an important role in the regulation of specific immunity and bone turnover. RANK Receptor was originally identified as a dendritic-cell-membrane protein, which by interacting with RANKL augments the ability of dendritic cells to stimulate na ve T cell proliferation and to promote the survival of RANK+ T cells. RANK is also expressed in a variety of tissues including skeletal muscle, thymus, liver, colon, small intestine and adrenal gland. The RANK/RANKL interaction is important in the regulation of osteoclastogenesis and in dendritic-cell mediated T cell immune responses. Impairments in RANK signaling have been implicated in the induction of expansile osteolysis and Paget disease of bone (PDB2) . Recombinant human sRANK receptor is a 19.1kDa polypeptide containing the TNFR homologous cysteine rich portion of the extracellular domain of RANK receptor.

 名称缩写/Abbreviation：

rHusRANK Receptor/TNFRSF11A

货号/Item No.：

UN408

别名/Synonyms：

Osteoclast differentiation factor receptor (ODFR)，CD256

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 19.1 kDa, a single non-glycosylated polypeptide chain containing 174 amino acids.

SCI的检索号/Accession #：

Q9Y6Q6 Gln29-Lys202

蛋白序列/A.A. Sequence：

QIAPPCTSEKHYEHLGRCCNKCEPGKYMSSKCTTTSDSVCLPCGPDEYLDSWNEEDKCLLHKVCDTGKALVAVVAGNSTTPRRCACTAGYHWSQDCECCRRNTECAPGLGAQHPLQLNKDTVCKPCLAGYFSDAFSSTDKCRPWTNCTFLGKRVEHHGTEKSDAVCSSSLPARK

纯度/Purity：

＞98% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. The ED₅₀ as determined by its ability to inhibit sRANKL induced nuclear factor kappa B(NF-κB) in RAW 264.7 cells is less than 50 ng/ml, corresponding to a specific activity of ≥ 2.0×10⁴ IU/mg in the presence of 15 ng/ml of recombinant sRANKL.

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris-HCl, 150 mM NaCl, pH 8.0.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.1 EU/μg of rHusRANK Receptor/TNFRSF11A protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.