Recombinant Human Monocyte Chemotactic Protein-4/CCL13

名称：Recombinant Human Monocyte Chemotactic Protein-4/CCL13

货号：UN176

规格：5μg/20μg/50μg/100μg/1mg

纯度：>96%

系统：E.coli.

产品详情

 Recombinant Human Monocyte Chemotactic Protein-4/CCL13

Human CCL13 is belonging to the CC chemokine family and is encoded by the gene CCL13 in humans. CCL13 (MCP-4) shares 56-61% sequence identity with MCP-1 (CCL2) and MCP-3 (CCL7) and is 60% identical to Eotaxin (CCL11) . CCL13 was a potent chemoattractant for monocytes and eosinophils and stimulated histamine release from basophils. CCL13 can induce a calcium flux in HEK-293 cells transfected with the receptor CCR2B and CCR3. That shows the function receptors of CCL3 are CCR2B and CCR3. Recombinant human MCP-4/CCL13 is an 8.6kDa protein containing 75 amino-acid residues including the four highly conserved cysteine residues present in the CC chemokines.

 名称缩写/Abbreviation：

rHuMCP-4/CCL13

货号/Item No.：

UN176

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 8.6 kDa, a single non-glycosylated polypeptide chain containing 75 amino acids.

SCI的检索号/Accession #：

Q99616 Gln24-Thr98

蛋白序列/A.A. Sequence：

QPDALNVPSTCCFTFSSKKISLQRLKSYVITTSRCPQKAVIFRTKLGKEICADPKEKWVQNYMKHLGRKAHTLKT

纯度/Purity：

＞96% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration of 10-100 ng/ml.

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, with 150 mM NaCl, pH 7.4.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.1 EU/μg of rHuMCP-4/CCL13 protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.