Recombinant Human Macrophage Inflammatory Protein-1 alpha/CCL3

名称：Recombinant Human Macrophage Inflammatory Protein-1 alpha/CCL3

货号：UN168

规格：5μg/20μg/50μg/100μg/500μg/1mg

纯度：>97%

系统：E.coli.

产品详情

 Recombinant Human Macrophage Inflammatory Protein-1 alpha/CCL3

CCL3, also known as macrophage inflammatory protein-1α (MIP-1α) , is a cytokine belonging to the CC chemokine family that is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes. Studies showed CCL3 can bind to CCR1, CCR4 and CCR5. MIP-1α can induce a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV) . Human MIP-1α shares approximately 73%, 74% and 92% amino acid sequence homology with the murine, rat and rhesus macaque protein Both human and murine MIP-1α and MIP-1β are active on human and murine hematopoietic cells. Recombinant Human MIP-1α is a 7.8kDa protein containing 70 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

 名称缩写/Abbreviation：

rHuMIP-1α/CCL3

货号/Item No.：

UN168

别名/Synonyms：

G0/G1 switch regulatory protein 19-1, MIP-1-alpha, PAT 464.1, SIS-beta, Small-inducible cytokine A3, Tonsillar lymphocyte LD78 alpha protein

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 70 amino acids.

SCI的检索号/Accession #：

P10147 Ala23-Ala92

蛋白序列/A.A. Sequence：

ASLAADTPTACCFSYTSRQIPQNFIADYFETSSQCSKPGVIFLTKRSRQVCADPSEEWVQKYVSDLELSA

纯度/Purity：

＞97% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 1.0-10 ng/ml.

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, with 150 mM NaCl, pH 7.4.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.01 EU/μg of rHuMIP-1α/CCL3 protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.