Recombinant Human MCP-2/CCL8

名称：Recombinant Human MCP-2/CCL8

货号：UN174

规格：2μg/10μg/50μg/100μg/500μg/1mg

纯度：>97%

系统：E.coli.

产品详情

 Recombinant Human MCP-2/CCL8

Human CCL8, also known as monocyte chemotactic protein 2 (MCP-2) , is belonging to the CC chemokine family hat signal through CCR2 and, with the exception of MCP-1, other CCR receptors. It is encoded by the gene CCL8. MCP-2 has two homogeneous MCP-1 (CCL2) and MCP-3 (CCL7) . These three MCPs were found by IL-1- beta triggered human MG-63 osteosarcoma cells. CCL8 shares 62 % amino acid sequence identity with MCP-1, and shares 58 % amino acid sequence identity with MCP-2. CCL8 has chemotactic function for monocytes, eosinophils and neutrophils. In addition, it can also chemoattract activated NK cells but not monocytes or neutrophils. The MCP family cross-reacts across species. Recombinant Human MCP-2 (CCL8) is an 8.9kDa protein containing 76 amino acid residues including the four highly conserved cysteine residues present in the CC chemokines.

 名称缩写/Abbreviation：

rHuMCP-2/CCL8

货号/Item No.：

UN174

别名/Synonyms：

HC14, Small-inducible cytokine A8

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 8.9 kDa, a single non-glycosylated polypeptide chain containing 76 amino acids.

SCI的检索号/Accession #：

P80075 Gln24-Pro99

蛋白序列/A.A. Sequence：

QPDSVSIPITCCFNVINRKIPIQRLESYTRITNIQCPKEAVIFKTKRGKEVCADPKERWVRDSMKHLDQIFQNLKP

纯度/Purity：

＞97% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-100 ng/ml.

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, with 150 mMNaCl, pH 7.4.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.01 EU/μg of of rHuMCP-2/CCL8 protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.