Recombinant Human Leptin

名称：Recombinant Human Leptin

货号：UN567

规格：200μg/1mg

纯度：>97%

系统：E.coli.

产品详情

 Recombinant Human Leptin

Encoded by the ob (obese) gene, Leptin is an adipose-derived cytokine that suppresses appetite and increases thermogenesis. The absence of leptin (or its receptor) leads to uncontrolled food intake and resulting obesity. Leptin exerts its anorectic effect via signaling through a hypothalamic receptor termed OB-R. Leptin has been shown to reduce body weight, food consumption, and plasma glucose levels in various in vivo models. Recombinant Human Leptin is a 16.0kDa protein containing 146 amino acid residues.

 名称缩写/Abbreviation：

rHuLeptin

货号/Item No.：

UN567

别名/Synonyms：

Obesity protein, OB

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 16.0 kDa, a single non-glycosylated polypeptide chain containing 146 amino acids.

SCI的检索号/Accession #：

P41159 Val22-Cys167

蛋白序列/A.A. Sequence：

VPIQKVQDDTKTLIKTIVTRINDISHTQSVSSKQKVTGLDFIPGLHPILTLSKMDQTLAVYQQILTSMPSRNVIQISNDLENLRDLLHVLAFSKSCHLPWASGLETLDSLGGVLEASGYSTEVVALSRLQGSLQDMLWQLDLSPGC

纯度/Purity：

＞97% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. The ED₅₀ as determined by a chemotaxis bioassay using human Leptin R transfected BaF3 murine proB cells is less than 2 ng/ml, corresponding to a specific activity of ≥ 5.0×10⁵ IU/mg

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in 50 mM PB, with 0.02% (v/v) Tween-20, pH 3.5.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.01 EU/μg of rHuLeptin protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.