Recombinant Human Interleukin-5

名称：Recombinant Human Interleukin-5

货号：UN079

规格：2μg/10μg/50μg/100μg/500μg/1mg

纯度：>98%

系统：E.coli.

产品详情

 Recombinant Human Interleukin-5

The IL-5 gene is located on chromosome 5 in humans, in close proximity to the genes encoding IL-3, IL-4, and granulocyte macrophage colony-stimulating factor, which are often co-expressed in TH2 cells. Through binding to the IL-5 receptor, IL-5 stimulates B cell growth and increases immunoglobulins secretion. IL-5 is a hematopoietic growth factor that stimulates the proliferation and activation of eosinophils. Produced by mast cells, T cells, and eosinophils, IL-5 plays an important role in inducing cell-mediated immunity against parasitic infections and certain tumors. Elevated levels of IL-5 lead to Eosinophilia, which may result in the induction of asthma and other allergic diseases. Human and murine IL-5 is cross-species reactive. Recombinant Human IL-5 is a 26.5kDa disulfide-linked homodimeric protein containing two 116 amino acid chains.

 名称缩写/Abbreviation：

rHuIL-5

货号/Item No.：

GH079

别名/Synonyms：

B-cell differentiation factor I, Eosinophil differentiation factor, TRF

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 26.5 kDa, a disulfide-linked homodimeric protein containing two 116 amino acids polypeptide chains.

SCI的检索号/Accession #：

P05113 Ile20-Ser134 with a N-terminal Met

蛋白序列/A.A. Sequence：

MIPTEIPTSALVKETLALLSTHRTLLIANETLRIPVPVHKNHQLCTEEIFQGIGTLESQTVQGGTVERLFKNLSLIKKYIDGQKKKCGEERRRVNQFLDYLQEFLGVMNTEWIIES

纯度/Purity：

＞98% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using human TF-1 cells is less than 0.1 ng/ml, corresponding to a specific activity of ≥ 1.0×10⁷ IU/mg.

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, with 3% (v/v) Trehalose, 0.1% (v/v) Tween-20, pH 7.5.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.01 EU/μg of rHuIL-5 protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.