Recombinant Human Interleukin-22

名称：Recombinant Human Interleukin-22

货号：UNO90

规格：2μg/10μg/50μg/100μg/500μg/1mg

纯度：>97%

系统：E.coli.

产品详情

 Recombinant Human Interleukin-22

IL-22 is a member of the IL-10 family of regulatory cytokines, which includes IL-10, IL-19, IL-20, IL-22, IL-24 and IL-26. Members of this family share partial homology in their amino acid sequences, but they are dissimilar in their biological functions. The human IL-22 gene is localized to chromosome 12q15. IL-22 has been shown to activate STAT-1 and STAT-3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal T cells upon anti-CD3 stimulation in humans. The functional IL-22 receptor complex consists of two receptor subunits, IL-22R (previously an orphan receptor named CRF2-9) and IL-10R beta (previously known as CRF2-4) , belonging to the class II cytokine receptor family. Mature human IL-22 protein shares approximately 79% aa sequence identity with mouse IL-22. Recombinant Human IL-22 is a 33.6kDa, non-disulfide-linked, homodimeric protein consisting of two 147 amino acid polypeptide chains.

 名称缩写/Abbreviation：

rHuIL-22

货号/Item No.：

UN090

别名/Synonyms：

nterleukin-22, IL-TIF, Cytokine ZCYTO18

表达系统/Source：

Expressed in E. coli.

分子量/Molecular Weight：

Approximately 33.6 kDa, a non-disulfide-linked, homodimeric protein consisting of two 147 amino acid polypeptide chains.

SCI的检索号/Accession #：

Q9GZX6 Ala34-Ile179

蛋白序列/A.A. Sequence：

MAPISSHCRLDKSNFQQPYITNRTFMLAKEASLADNNTDVRLIGEKLFHGVSMSERCYLMKQVLNFTLEEVLFPQSDRFQPYMQEVVPFLARLSNRLSTCHIEGDDLHIQRNVQKLKDTVKKLGESGEIKAIGELDLLFMSLRNACI

纯度/Purity：

＞97% by SDS-PAGE or HPLC.

活性/Biological Activity：

Fully biologically active when compared to standard. The ED₅₀ as determined by inducing IL-10 secretion of human COLO 205 cells is less than 0.3 ng/ml, corresponding to a specific activity of ≥ 3.3×10⁶ IU/mg.

配方/Formulation：

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, with 150 mM NaCl, pH 5.0.

外观/Appearance：

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：

< 0.01 EU/μg of rHuIL-22 protein as determined by LAL method.

复溶/Reconstitution：

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.