Recomb inant Human Insulin-like Growth Factor-1

名称：Recomb inant Human Insulin-like Growth Factor-1

货号：UN037

规格：10μg/100μg/500μg/1mg

纯度：>98%

系统：E.coli

产品详情

Recombinant Human Insulin-like Growth Factor-1

The insulin-like growth factors (IGFs) belonged to the insulin gene family, are mitogenic polypeptide growth factors that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. The IGFs are similar by structure and function to insulin, but have a much higher growth-promoting activity than insulin. IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. The production of IGF-1 is stimulated by growth hormone (GH) and can be retarded by undernutrition, growth hormone insensitivity, lack of growth hormone receptors, or failures of the downstream signaling pathway post GH receptor including SHP2 and STAT5B. Mature human IGF-1 shares 94% and 96% aa sequence identity with mouse and rat IGF-1, respectively , and exhibits cross-species activity. Recombinant human IGF-1 is a non-glycosylated polypeptide chain containing   70 amino acids..

名称缩写/Abbreviation：  

rHuIGF1

货号/Item No.：  

UN037

别名/Synonyms：  

Somatomedin C, IGF-I, IGF-IA, Mechano growth factor, MGF

表达系统/Source：  

Expressed in E. coli.

分子量/Molecular Weight：  

Approximately 7.6 kDa, a single non-glycosylated polypeptide chain containing 70 amino acids.

SCI的检索号/Accession #：  

P05019 Gly49-Ala118

蛋白序列/A.A. Sequence：  

GPETLCGAELVDALQFVCGDRGFYFNKPTGYGSSSRRAPQTGIVDECCFRSCDLRRLEMYCAPLKPAKSA

纯度/Purity：  

＞98% by SDS-PAGE or HPLC.

活性/Biological Activity：  

Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2 ng/ml, corresponding to a specific activity of ≥ 5.0×10⁵ IU/mg.

配方/Formulation：  

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PBS, pH 7.0.

外观/Appearance：  

Sterile filtered White lyophilized (freeze-dried) powder.

内毒素/Endotoxin：  

< 0.01 EU/μg of rHuIGF1 protein as determined by LAL method.

复溶/Reconstitution：  

Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

稳定性和存储/Stability & Storage：  

For long term storage, the product should be stored ≤ -20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 36 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution.

* THIS PRODUCT IS FOR RESEARCH, LABORATORY AND EVALUATION PURPOSE ONLY.